Signal-sequence-independent secretion of the staphylococcal nuclease in Mycobacterium smegmatis
نویسندگان
چکیده
منابع مشابه
Signal-sequence-independent secretion of the staphylococcal nuclease in Mycobacterium smegmatis.
Staphylococcus aureus nuclease is a small, secreted protein which has been successfully used as a reporter system to identify exported products in Lactococcus lactis. Here, biochemical evidence is provided that the nuclease is exported by Mycobacterium smegmatis in the presence, but also in the absence of a signal sequence, and thus probably independently of the Sec translocation pathway. This ...
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متن کاملComplementation of the Lactococcus lactis secretion machinery with Bacillus subtilis SecDF improves secretion of staphylococcal nuclease.
Unlike Bacillus subtilis and Escherichia coli, the gram-positive lactic acid bacterium Lactococcus lactis does not possess the SecDF protein, a component of the secretion (Sec) machinery involved in late secretion stages and required for the high-capacity protein secretion in B. subtilis. In this study, we complemented the L. lactis Sec machinery with SecDF from B. subtilis and evaluated the ef...
متن کامل[Cold denaturation of staphylococcal nuclease].
It has been shown that the structure of staphylococcal nuclease breaks down reversibly both at a temperature increase above 20 degrees C and at its decrease. Both the heat and cold denaturations of protein are well approximated by a transition between two states differing in heat capacity, which means that the whole protein molecule represents a unique cooperative system with a well developed h...
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ژورنال
عنوان ژورنال: Microbiology
سال: 2002
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-148-2-529